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Speakers - Quezia Bezerra Cass

Type of the Presentation: (Short lecture)

NEW APPROACHES IN LABEL-FREE SCREENING ASSAYS

 

Kenia L. Vanzolini, Adriana Miranda dos Santos, Dulce Helena Ferreira de Souza, Quezia Bezerra Cass.

 

Chemistry Department, Separare, Federal University of São Carlos, SP, Brazil,

Correspondence: quezia@pq.cnpq.br

 

To select how to profile a target bioactive compound is not an easy task, either from a synthetic or a natural combinatory library. The screening approach must have some important characteristic, as relevant and effectiveness results, without false-positive or negative. Additionally to this, the assay has to be fast, robust, precise and accurate. In this respect, affinity-based screening assays emerged as a useful tool for monitoring binding events aiming the isolation, identification and/or characterization of bioactive compounds. The identified hit can be fully characterized in regarding to affinity towards the target protein. A variety of approaches based either on zonal or frontal bioaffinity chromatography can be explored.1 For this presentation, a critical overview of the recently emerged ligand-fishing assay will be sightseen for profiling crude aqueous natural product extracts. The results obtained indicate the selectivity of the enzyme against a complex sample, fishing out just the compounds with highest affinity.  The use of zonal chromatography for the kinetics and screening assays of two native acetylcholinesterase of Atta. sexdens will be presented and fully discussed.