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Abstracts - Waleria Ferreira Rabelo

Type of the Presentation: Poster

STUDY OF CHANGE IN CHEMICAL PROFILE OF SEEDLINGS GENOTYPES FROM CITRUS WHEN EXPOSED TO OMICETO Phytophthora nicotianae.

Waleria Ferreira Rabelo*1, Luis G. C. Durango1, João Batista Fernades1, Paulo Cezar Vieira1, Moacir Rossi Forim1 and Maria Fatima G. Fernandes da Silva1

1 Dept. of Chemistry, Federal University of São Carlos.

*e-mail: wallrabelo@hotmail.com

The root rot caused by Phytophthora nicotianae, is a leading in citrus and is widespread in all citrus regions of the world. The selection of resistant genotypes and / or tolerant to the disease, is an important step in the improvement of citrus and comparative studies programs as to their chemical profile of the interaction between the pathogen and the plant where the reaction of the organism attack ranges from its very high susceptibility to its high strength. The present work describes the study of variation in the chemical profile of citrus after inoculation of "Phytophthora nicotianae", responsible for gomose disease, that is one of the most serious diseases that cause damage in Brazilian citrus. The omiceto “P.nicotianae” was inoculated in seedlings of the "Valencia" (Citrus sinensis (L.) Osbeck) grafted on "Citrumelo Swingle" (Poncirus trifoliata x Citrus paradisi), and was evaluated the variation of some secondary metabolites by the development of analytical methods such as HPLC-UV, LCMS/MS and chemometric tools. These chemometric methods applied to the data obtained from studies UHPLC-MS/MS allowed to discriminate plants infected with the bacteria against the healthy ones. By analyzing the variation in the chemical profile of citrus studied by UHPLC –MS/MS was possible to detect the variation of some coumarins mainly in the roots and stem.