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Abstracts - Viviane de Cassia Pereira Abdalla

PHYTOTOXIC POTENTIAL OF MYRICITRIN FROM MYRCIA SPLENDENS (Sw.) DC. MYRTACEAE

Flávia Cevithereza Pontes1 Viviane de Cassia Pereira Abdalla1*, Carolina Rabal Biasetto2, Maristela Imatomi1, Alberto José Cavalheiro2 and Sonia Cristina Juliano Gualtieri1

1 Botany Department, Federal University of São Carlos; vivicpereira@gmail.com; maristelaimatomi@yahoo.com.br; soniacristina3012@hotmail.com

2 Organic Department, São Paulo State University, UNESP, Chemistry Institute, Araraquara, SP- Brazil;  albjcava@iq.unesp.br; carolinarabal@yahoo.com.br

* Correspondence: vivicpereira@gmail.com

Myrcia splendens (Sw.) DC. (Myrtaceae) is a tree species found in the Brazilian cerrado and there are few studies that investigate the phytotoxic potential of its secondary metabolites. Research evaluating the bioactivity of the compounds is important for discovering new substances that have some kind of action on the development of other invasive plants. This study evaluated the phytotoxic activity of myricitrin, a compound isolated from leaves of M. splendens. Liquid-liquid partitions were made using dichloromethane and methanol from 50g of dried leaves of M. splendens. Later the extract was fractionated and the fractions were tested on the growth of wheat coleoptiles (Triticum aestivum)1. Because it was more promising in the bioassay of wheat coleoptile growth, the ethyl acetate fraction was refracted and tested again. FM8 subfraction was active on coleoptile growth. This was purified by HPLC techniques and identified by NMR and LC-MS analyzes. Thus it was possible to isolate and identify the compound myricitrin. This was tested on the growth of wheat coleoptiles and on the growth of colony grass seedlings (Megathyrsus maximus). The results indicate that this compound inhibited the growth of wheat coleoptiles at molar concentrations of 10-3; 3.10-4 and 10-4M showing inhibition percentages of 27%, 26% and 26%, respectively. Controls were made with buffer solution and with the herbicide oxyfluorfen at the same concentrations. The growth average of the shoot of the M. maximus was affected by myricitrin in the concentrations 10-3 and 3.10-4 M when compared with the negative control (buffer solution), indicating who this compound is phytotoxic to this invasive plant in that concentrations. The root was not affected.

Refs.

1. Macías, F.A. et al., J. Chem. Ecol.36, 4, 2010.