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Abstracts - Vicente Machado

Type of the Presentation: Oral presentation

ATTRACTIVENESS OF 1-HEXANOL TO Nyssomyia neivai (DIPTERA: PSYCHODIDAE) IN DIFFERENTS STAGES OF THEIR LYFE CYCLE

Vicente Machado1*, Flávia Silva2, Wanderson Oliveira3, and Mara Pinto4

1 Faculdade de Ciências Farmacêuticas de Araraquara; vicentemachado@yahoo.com.br

2 Faculdade de Ciências Farmacêuticas de Araraquara; flavinhabenini@gmail.com

3 Faculdade de Ciências Farmacêuticas de Araraquara; wanderson.henrique.unesp@gmail.com

4 Faculdade de Ciências Farmacêuticas de Araraquara; marap@fcfar.unesp.br

* Correspondence: vicentemachado@yahoo.com.br

Sand flies are incriminated in the transmission of several etiological agents, being the most important in public health the group of Leishamnia spp which may cause the cutaneous and visceral Leishmaniasis. The sand fly Nyssomyia neivai is important in the transmission of the etiological agent of cutaneous leishmaniasis in Brazil. Light traps are the usual method for monitoring those insects, however there are some species that are not phototropic. Moreover, light attracts many other insects and it is very difficult to separate the tiny sand flies from other insects inside the traps. Therefore the search of volatile compounds attractive for sand flies are interesting. Besides, it is relevant to evaluate the attractiveness of the compound in different physiological stages of their lives. The aim of the present study was to evaluate the attractiveness of the compound 1-hexanol to Ny. neivai females in three physiological stages: between 4-7 days of life; 2 days after the blood meal (6-9 days of life) and gravid females (7-10 days of life) and males (4-7 days of life). We tested 2 mL of 1-hexanol in a release system (in process of patenting). The tests were carried out in a wind tunnel. Activation (number of insects that leave the release chamber) and attraction (number of insects that reach the stimulus) behaviors were evaluated for two minutes. For the control group we used the diluent hexane. For males 29/30 were activated and 28/30 were attracted and for the control 2/30 were activated and 1/30 were attracted. For females (4-7 days of life) 30/30 were activated and 29/30 were attracted and for the control 3/30 were activated and 3/30 were attracted. For females (after blood meal) for the compound and the control 0/30 were activated and 0/30 were attracted. For gravid females 20/30 were activated and 14/30 were attracted and for the control 1/30 were activated and 1/30 were attracted. Among the evaluated groups, only the females after the blood meal did not show any responses for the compound. Females (4-7 days) and males were strongly activated and attracted for the 1-hexanol (p<0.05). Gravid females presented intermediate responses of attractiveness. The results clearly evidence that the physiological stage interferes in the attractiveness of sand flies.

Financial support: CNPq