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Abstracts - Rita Cássia N. Pedroso

LIGHT QUALITY ON FLAVONOID PRODUCTION AND GROWTH IN VITRO OF Hyptis marrubioides SEEDLINGS

Rita Cássia N. Pedroso1, Núbia Angélica A. Branquinho2, Alessandra C.B.A.M. Hara2, Alan Carlos Costa2, Fabiano Guimarães Silva2, Letícia P. Pimenta1, Márcio Luiz A. Silva1, Wilson Roberto Cunha1, Patrícia M. Pauletti1, Ana Helena Januário1

1 Center for Research in Exact and Technological Sciences, University of Franca. Av. Dr. Armando Salles de Oliveira, 201, 14404-600, Franca, São Paulo, Brazil.

2 Federal Institute of Education, Science and Technology Goiano, Campus Rio Verde,

Rio Verde, GO, Brazil

* Correspondence: rita-pedroso@hotmail.com.br

The genus Hyptis, Lamiaceae, comprises about 300 species, widely distributed, occurring mainly in tropical regions of the Americas and Africa. Hyptis marrubioides Epling, commonly known as mint-of-field, is a species of the Brazilian Cerrado traditionally used to treat gastrointestinal and skin infections, pain, and cramps.1 The aim of the present study was to assess the effect of light quality on growth of H. marrubioides seedlings in vitro and their flavonoid production. Hyptis marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m−2s−1irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465

± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g−1of dry weight) and blue lights (0.298 mg g−1of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro- cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation.

Refs.

1. McNeil, M., Facey, P., Porter, R. Essential oils from the Hyptis genus – a review (1909–2009). Nat. Prod. Commun. 6, 1775–1796, 2011.