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Abstracts - Miryan D. A. Coracin

MALE AND FEMALE ATTRACTIVENESS OF GYROPSYLLA SPEGAZZINIANA (HEMIPTERA: PSYLLIDAE) TO FEMALE SEX PHEROMONE

 

Adriana H. Walerius1*, Ramona dos S. Bandeira 1, Diogo M. Vidal2, Daiane Szczerbowski2 and Miryan D. A. Coracini1

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1  Universidade Estadual do Oeste do Paraná, Rua Universitária 2069, Jardim Universitário, 85819-110 Cascavel, PR, Brasil. adrianahelenawalerius@gmail.com, bandeira.ramona@gmail.com, miryan.coracini@unioeste.br

2  Universidade Federal do Paraná, Departamento de Química, Laboratório de Semioquímicos, Centro Politécnico, Jardim das Américas, 81531-990 Curitiba, PR, Brasil. vidal@qui.ufmg.br, daianeszcz@yahoo.com.br

*  Correspondence: miryan.coracini@unioeste.br

 

Insects use signals for interspecific communication, which can be visual, acoustic and chemical signals. Sex pheromones are the main intermediaries in mating. In the superfamily Psylloidea (Hemiptera) there are only studies about four species that use chemical signals to locate the co-specific. Therefore, the aim of this study was to verify the attractiveness of males and females of the yerba mate psyllid, Gyropsylla spegazziniana (Hemiptera: Psyllidae), in bioassays with an Y-olfactometer, and to identify the sexual pheromone of this species. In the bioassays it was used live insects (virgin males and females) in a branch of yerba mate as odor source against the control, an empty branch. Previous behavior tests showed that without the yerba mate branch the insects do not mate, and the female needs to walk and place itself at the tip of the branch to start the mating behavior; the male will walk towards the female and mate. In order to analyze the presence of the sex pheromone, body extracts of virgin males and females were done, which were analyzed in a gas chromatograph coupled to the mass spectrometer (GC-MS). As a result, males (63%) and females (67%) were attracted to live females in the olfactometer, but were not attracted to live males, suggesting the presence of volatiles released by females for sexual attraction. It was found the presence of a female-specific compound, identified as 2-nonadecanone, as a possible sex pheromone. Thus, the results show that females of G. spegazziniana release sex pheromone to attract males for mating.

 

Keywords - Yerba mate, Y-olfactometer, pheromone extraction