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Abstracts - Mayara De Carli

Type of the Presentation: Poster

SEXUAL BEHAVIOR OF Diabrotica viridula (COLEOPTERA: CHRYSOMELIDAE)

Mayara De Carli1*, Caroline Rech1, Arodí Prado Favaris2, José Maurício Simões Bento2 and Cristiane Nardi1

1 Department of Agronomy, Midwestern State University, 85040-080, Guarapuava, Paraná, Brazil

2 Department of Entomology and Acarology, University of São Paulo, USP/ESALQ, 13418-900, Piracicaba, São Paulo, Brazil

* Correspondence: decarlimayara@gmail.com

Diabrotica viridula F. (Coleoptera: Chrysomelidae) is currently reported as pest in maize, Zea mays L., in Brazil and other countries of South America. Despite of that, few studies have evaluated bioecological and behavioral aspects of this insect to establish a suitable pest management program. Here, we investigated the age and hour of the first mating of D. viridula couples (n=40), and using an olfactometer, we measured the behavioral responses of both sexes (n=40) to odors sources emitted by males and females extracts obtained by push-pull system. Also, we assessed the electroantennographic response of males and females those extracts (N=10). The results revealed that first mating in D. viridula occurred on the third day after emergence, especially during the scotophase. Behavioral assays provide that males were attracted to emanations from conspecific females, and male antennae showed two high response to extract of them. These findings suggest that D. viridula females produce a sex pheromone, which consist of two active chemical compounds. Further studies are ongoing to identify and synthesize this sex pheromone to evaluate their field use.

Acknowledgement: The authors acknowledge the financial support from National Institute of Science and Technology – Semiochemicals in Agriculture (FAPESP and CNPq -– grants #2014/50871-0 and #465511/2014-7, respectively) and Fundação Araucária.