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Abstracts - Marina Zequin

CHEMICAL STUDY OF Hortia oreadica FLOWERS 

Marina Zequin1*, Maria Fátima G. F. Silva1, João Batista Fernades, Paulo Cezar Vieira and Moacir Rossi Forim


1 UFSCAR; marinazequin@gmail.com
2 UFSCAR; dmfs@ufscar.br
* Correspondence: marinazequin@gmail.com

INTRODUCTION
Previous studies of H. oreadica showed a large diversity of secondary metabolites in diverse parts of the plant, so a chemical study of the H. oreadica (Rutaceae) flowers
was made to evaluate if this variety of metabolites remains in the extremities of this plant.

MATERIAL AND METHODS
The hexane extract was passed through a column of silica (230-400 mesh, column size: 3,5 x 33 cm) and eluted with Hex:AcOEt:MeOH resulting in five
fractions that was analyzed by Thyn-Layer chromatography, and the first fraction seemed to be more interesting. This fraction was submitted to chromatography in Silica
(230-400 mesh, column size: 3,5 x 25 cm) and eluted with Hex:AcOEt:MeOH, which afforded the isolation and identification of the compound 1 by NMR 1D and 2D (1H,
13C, HSQC, HMBC and COSY).
RESULTS AND DISCUSSION
The NMR data of 1H and 13C are presented in table 1.
In comparison of literature data, the compound 1 was identified as 3α-hydroxytirucalla-7,24-dien-21-oic acid1.
This compound already demonstrates several biologicals activities.

Refs.
1. Takashi, K. et al., Chemistry and Biodiversity, 9, 1500, 2012