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Abstracts - Luize Zola Ramin

A GREEN ALTERNATIVE TO EXTRACT COMPOUNDS WITH ANTIMICROBIAL POTENTIAL FROM ORANGE WASTE

Luize Zola Ramin1, Danielle Francischini1, Karine Zanotti1, Moacir Rossi Forim1, João Batista Fernandes1, Maria Fátima Graças Fernandes Silva1, Vânia Gomes Zuin1*

1 Federal University of São Carlos - São Carlos, 13565-905, Brazil

* Correspondence author: vaniaz@ufscar.br

Several researches have shown that orange waste can be a source of high value products, generating profits and increasing the industry’s competitiveness 1. This biomass may contain interesting compounds, such as soluble sugars, cellulose and hemicellulose, pectin, D-limonene and flavonoids. The last compounds have being reported as a potential source of natural antimicrobials which are active against Gram-negative bacteria 2. The main purpose of this study is to present an evaluation about chemical composition of real citrus waste. Focusing on the secondary metabolites, by the phytochemical screening, it is possible to target the most attractive option to use as a renewable source of phenolic compounds within this biomass.

The extraction of phenolic compounds was performed with ultra-turrax technique, using ethanol as solvent extractor. In order to achieve the best conditions for this extraction, it was proposed a factorial design 24 evaluating time of extraction, solvent concentration, solvent/sample ratio and ultra-turrax rotation.

Many different flavonoids derived from such matrices have been separated and identified using reversed phase HPLC-UV. Four flavonoids were used as markers (hesperidin, naringenin, hesperetin and nobiletin) and, through standardized samples, it was possible to identify and quantify some phenolic compounds. By optimizing the extraction, it was possible to extract significant amount of these flavonoids, mainly hesperidin, known as the major compound of this matrix.

Ref.

1. Zuin, V.G. Pure Appl. Chem., 88, 2016.

2. Mandalari, G., et al. Journal of Applied Microbiology, 103, 6, 2007.

Acknowledgements

Fapesp and Capes (13/12052-5; 2032/2014-07; 14/50827-1)