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Abstracts - Flávia Silva

COMPARISON OF VOLATILES EMITTED BY BALB/C MICE INFECTED WITH Leishmania braziliensis (KINETOPLASTIDA: TRYPANOSOMATIDAE) AND NOT INFECTED

Flávia Silva1*, Vicente Machado2, Wanderson Oliveira3, Danilo Miguel4, Christiann Tosta5 and Mara Pinto6

1 Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho” (FCFAr/UNESP); flavinhabenini@gmail.com

2 Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho” (FCFAr/UNESP); vicentemachado@yahoo.com.br

3 Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho” (FCFAr/UNESP); wanderson.henrique.unesp@gmail.com

4 Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP); danciccone@gmail.com

5 Instituto Federal de Educação, Ciência e Tecnologia de São Paulo (IFSP), Câmpus Matão; cdtosta@yahoo.com.br

6 Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista “Júlio de Mesquita Filho” (FCFAr/UNESP); marap@fcfar.unesp.br

* Correspondence: flavinhabenini@gmail.com

Kairomones are volatiles compounds released by some organisms and trigger a beneficial response to other species. These compounds act as important chemical cues used by hematophagous insects to locate their hosts. Some studies have indicate that parasites are able of affect the volatiles released by their hosts and influence on attractiveness of vectors to them, like the etiological agents of malaria (Plasmodium spp) and visceral leishmaniasis (Leishmania infantum chagasi). For Leishmania braziliensis, etiological agent of cutaneous leishmaniasis, there still no evidences of such phenomenon. The aim of this study was to evaluate any differences on volatiles released by BALB/c mice infected or not with Leishmania braziliensis. To identify volatiles presented by the two groups of animals solid phase micro extraction (SPME) technique coupled with gas chromatography-linked mass spectrometry (GC-MS) was used. Each mice (infected and not infected) had 100 mg of hair collected from the dorsal region with a razor blade. Hairs were conditioned in a headspace vial of 20 mL. After standardization, a fiber coated with polydimethylsiloxane/divinylbenzene (PDMS/DVB) was inserted on the vial and exposed by 30 minutes for volatiles extraction, at 90 ºC. An empty vial was used as control. Fibers were inserted in GC-MS QP2010 Plus for analysis and the tests were carried out in triplicates. From the analysis of the mass spectrum, the compounds were identified by association of mass spectrum with libraries of GC-MS (NIST 08, NIST 98v101, Wiley MS 229 e FFNSC 1.3) and by calculating retention index (RI) with a mixture of C8–C20 n-alkanes. No differences were observed on the profile of volatiles released by the two groups of animals, with 38 compounds identified.

Financial support: FAPESP; CNPq.