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Abstracts - Eliane de O. Borges

ABDOMINAL SCENT GLANDS AND ALAR ANDROCONIA PHEROMONES OF HELICONIINAE MALES (LEPIDOPTERA; NYMPHALIDAE)

Eliane de O. Borges1, 3*, Daiana Bonfantti1, Douglas J. de Melo3, Camila B. da C. Martins3, Diogo M. Vidal2, Luana O. Hoffmann3 and Paulo H. G. Zarbin1, 3

1 Programa de Pós-graduação em Entomologia, Universidade Federal do Paraná, Departamento de Zoologia, C. P. 19020, CEP 81531-980, Curitiba, PR, Brasil. elianeoborges@gmail.com

2 Departamento de Química, Universidade Federal de Minas Gerais (UFMG), 31270-901, Belo Horizonte-MG, Brasil. vidal@qui.ufmg.br

3 Laboratório de Semioquímicos, Departamento de Química, Universidade Federal do Paraná (UFPR), 81531-980, Curitiba-PR, Brasil.

* Correspondence: elianeoborges@gmail.com

Lepidoptera of the Heliconius genus are known for association with passion vine. In Heliconiinae the interspecific communication through sex pheromones benefits the sexual encounter, the courtship, the protection of offspring and newly mated female. The abdominal scent glands (ASG) are present in male valves and possues volatiles with anti-aphrodisiac function what are transferred during copulation to the ASG of the female, an inflatable bag, displayed during the courtship behavior and defense situations. The androconia, set of differentiated scales that are used for the dispersion of male-specific pheromones. The androconial scales release pheromones during the courtship that would make the female predisposed to mating. The main objective of this research is to analyze the morphology, chemical composition, and ethology related to ASG and alar androconia of Heliconius erato phyllis, Heliconius ethilla narcaea and Heliconius besckei males. Morphological studies were conducted using stereomicroscopy, scanning electron microscopy and transmission electron microscopy. As methodology of analytical procedures were adopted the extraction with solvent, as chemical analysis method were adopted the gas chromatography, the gas chromatography associated to mass spectrometry and the infrared spectrometry. The methodology of analyze of the bioactivity of the compounds was made through electroantennography and behavioral bioassays. The morphology analyze of ASG showed that its possues diferentied structures que are possibily envolves in dispersion of volatiles in females. The androconial scales have a similar morphology in the specimens studied and showed internal structures possibily related whit the elimination of pheromone secretions and volatiles; and were identified the producing glands. Were found volatile bioactive compounds in ASG of H. erato phyllis and possibily bioative componds in H. ethylla narcaea and H. besckei. Possibily bioative componds were found too in androconia of H. erato phyllis, H. ethylla narcaea and H. besckei males. Bioassays with virgin females proved the anti-aphrodisiac function of odoriferous bouquet of H. erato phyllis male valve and showed that the compound (E)-3,7,11-trimetiloxacicloundec-7-en-2-ona is one male specific anti-aphrodisiac compound.