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Abstracts - Diego M. Magalhães

Type of the Presentation: Poster

OVIPOSITION BEHAVIOUR OF Anastrepha fraterculus (WIEDEMANN) (DIPTERA: TEPHRITIDAE) IN RESPONSE TO FECES EXTRACTS

Diego M. Magalhães1*, Miguel Borges2, Raúl A. Laumann3, Claudio de A. Barros4, Adalécio Kovaleski5 and Maria Carolina Blassioli-Moraes6

1 EMBRAPA Recursos Genéticos e Biotecnologia; magalhaes.dmm@gmail.com

2 EMBRAPA Recursos Genéticos e Biotecnologia; miguel.borges@embrapa.br

3 EMBRAPA Recursos Genéticos e Biotecnologia; raul.laumann@embrapa.br

4 EMBRAPA Uva e Vinho; claudio.barros@embrapa.br

5 EMBRAPA Uva e Vinho; adalecio.kovaleski@embrapa.br

6 EMBRAPA Recursos Genéticos e Biotecnologia; carolina.blassioli@embrapa.br

* Correspondence: magalhaes.dmm@gmail.com

The South American fruit fly, Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae), is one of the most destructive polyphagous pests in South America. Following oviposition, female flies deposit host marking pheromones (HMP) to indicate that the host fruit has already been infested. We examined the oviposition behaviour of A. fraterculus to artificial fruits (agar spheres wrapped in Parafilm) marked with aqueous and methanolic feces extracts. We also evaluated the effect of feces extracts from virgin and mated males and females, and from mated couples to determine a dose of fecal extracts that effectively reduce the degree of infestation in fruits. Aqueous extracts of fruit fly feces did not elicit deterrent effect in females (P>0.05). The same was observed for methanolic extracts of feces from virgin and mated males and virgin females (P>0.05). However, the methanolic extracts of feces from mated females (P<0.001) and mated couples (P<0.001) drastically reduced the number of laid eggs in the fruits. Artificial fruits treated with mated couples methanolic feces extracts at 50 insects equivalent (IE), 10 IE and 1 IE resulted in a significant reduction of infestation (P<0.001). This is the first time that methanolic feces extracts at 1 IE dose is reported to be oviposition deterrent in fruit fly species. Altogether, our results suggest that few amounts of HMP might effectively reduce fruit infestation by A. fraterculus, making possible the use of fecal extracts in orchards for management strategies. Chemical identification of HMP components is currently under investigation.