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Abstracts - Daniele F.O.Rocha

ENVIRONMENTAL ADAPTATION OF ANTARCTICA BACTERIAL ISOLATES INVESTIGATED BY PROTEIN FINGERPRINTING

Daniele F.O.Rocha1*, Tiago R. Silva2, Fabio N. dos Santos,1 Valeria M. Oliveira2 and Marcos N. Eberlin1.

1 Chemistry Institute, University of Campinas - UNICAMP, Campinas-SP, Brazil.

2 Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas - UNICAMP, Campinas, Brazil.

* Correspondence: drocha@iqm.unicamp.br

The extremophile organisms adapted to live in Antarctica adopted survival strategies to face the low temperatures, and adverse radiation, hydration and nutritional conditions. The environmental heterogeneity could justify the great microbial diversity in this continent, since the metabolic changes for these adaptations are taxa specific. Therefore, the production of unique biomolecules by Antarctic microorganisms has been very attractive for chemical ecology and bioprospecting studies. Protein fingerprinting by MALDI-MS is known for its ability for microbial classification in a simple method for sample preparation and data collection. The sensitivity of this approach goes beyond taxonomic purposes revealing also host and environmental adaptation. In this work, we confirmed the power of this approach to indicate metabolic changes caused by environmental stresses in polar environment. We investigated 30 bacterial isolates collected in different locations of Antarctica by means of protein extraction with acetonitrile/formic acid solution, followed by MALDI-TOF-MS analysis between m/z 2 and 20,000. The taxonomic classification by HCA was obtained in MetaboAnalyst when considered the isolates’ origin, by dividing the dataset into subgroups of soil biofilm, sediment and marine animal sampling. For Arthrobacter antarticus the environmental influence is greater than taxonomic similarities since strains collected from soil and marine animals were discriminated in PLS-DA and HCA. Given the heterogeneity of environmental conditions in Antarctica, MALDI-MS provides a simple and powerful resource to explore microbial diversity and adaptation caused by environmental stresses. These preliminary results revealed the participation of proteins in adaptation and survival in this fascinating continent, additionally serving for the screening of biomolecules related to these processes.

Refs.

  1. Braga, P. A. C. et al. RSC Adv. 2013, 3 (4), 994-1008.

  2. Kosina, M. et al., Current Microbiol. 2013, 67 (6), 637-646.

  3. Rocha, D. F. O. et al., Anal. Bioanal. Chem. 2017, 1-10.