Abstracts - Carolina R. Biasetto


Carolina R. Biasetto1*, Andressa Somensi1, Juliana R. Lopes1, Viviane de C. P. Abdalla2, Sonia C. J. Gualtieri2, Vanderlan da S. Bolzani1 and Angela R. Araujo1

1NuBBE - Nucleus of Bioassays, Biosynthesis and Ecophysiology of Natural Product, São Paulo State University, UNESP, Chemistry Institute, Araraquara, SP, Brazil;

2Botany Department, Federal University of São Carlos, São Carlos, SP, Brazil;


Eugenia brasiliensis (Myrtaceae) is a Brazilian species that produces small edible fruits with a sweet cherry-like flavor. Its wood is used in carpentry and woodworking. This specie has also been used in traditional medicine as anti-reumathic, diuretic and anti-inflammatory1.The endophytic fungi are microorganisms living asymptomatically in the intercellular space of host plants and establish a mutualistic relationship with its host during a part or all their life2. As part of our studies on bioactive metabolites produced by endophytic fungi, we chose E. brasiliensis for studies. Seventeen endophytic fungi were isolated from stem, leaves and ripe fruits using traditional methodology3. Among these, Xylaria cubensis, Diaporthe sp. and Colletotrichum sp. were chosen for detailed chemical investigation. These endophytic fungi were cultivated on PDB medium (large scale) for 28 days at 25 oC. The broth were separated from the mycelium by filtration and the crude extracts were obtained by extraction with EtOAc and dried in rotatory evaporator. The crude extracts were subjected to bioassays to evaluation of the antifungal, anticholinesterasic and cytotoxic activities and have shown promising results. The crude EtOAc extracts were fractionated by chromatographic techniques, affording in the isolation of several secondary metabolites. X. cubensis, Diaporthe sp. and Colletotrichum sp. are excellent producer of bioactive metabolites, with the production of cytochalasins, isocoumarins, diketopiperazines, among others, which are described with numerous important biological activities4-6. The structures were elucidated by analysis of 1H NMR and compared with the literature4-6. The cytochalasin D and C showed phytotoxic activity. These results reinforce the potential of these microorganisms as sources of bioactive natural products and corroborate the assumption of ecological interaction between the endophyte and its host plant, either by protecting it against pathogens and predators or by assisting in its physiological development.


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3. Helder, L. T. et al., Phytochemistry. 66, 2363-2367, 2007.

4. Oliveira, C. M. et al., Phytochemistry Letters. 4, 93-96, 2011.

5. Cafêu, M. C. et al., Quim. Nova. 8, 991-995, 2005.

6. Chapla, V. M.; Biasetto, C. R.; Araujo, A. R., Rev. Virtual Quim. 5, 421-437, 2013.