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Abstracts - Alexandre Jordão

ULTRASTRUCTURE OF ANTENNAE SENSILLA OF Hypsipyla ferrealis (HAMPSON) (LEPIDOPTERA: PYRALIDAE) AND CHEMICAL COMPOSITION OF THE FEMALE SEX GLAND

Merybeth Triana1*, Paulo França1, Johnnatan Freitas2, Jeniffer Freitas2, Alexandre Jordão3, Henrique Goulart1, Antônio Santana1

1Laboratory of Natural Products Research, Center of Agricultural Sciences, Federal University of Alagoas (CECA-UFAL), Maceio, Brazil; merybeth.triana@iqb.ufal.br

2Microscopy Laboratory, Federal Institute of Alagoas (IFAL), Maceio, Brazil; johnnatandf@gmail.com

3Laboratory of Ecosystems Entomology, Scientific and Technological Research Institute of Amapa State (IEPA), Macapa, Brazil; aljordao@hotmail.com

Seeds of the crabwood tree Carapa guianensis Aubl. (Meliaceae) are widely targeted by the pharmaceutical and cosmetic industries in Brazil. However, the potential of the oil production and its germination can be lost by damages to the seeds caused by the moth Hypsipyla ferrealis (Hampson) (Lepidoptera: Pyralidae). Therefore, studies in the sexual communication of H. ferrealis are fundamentals for the decrease in population density. The aim of this work is to perform a preliminary characterization of the morphology of the antennae and to analyze the chemical composition of the sex gland of H. ferrealis.

Antennae were viewed using scanning electron microscope and digital images were analyzed to determine each type of sensilla. Abdominal glands were excised from virgin females in calling behavior and were immediately immersed in hexane subsequently extract was analyzed by GC-FID, GC-EAD and GC-MS.

The antennae were divided into two main areas. The dorsal surfaces had two rows of scales. The ventral surfaces carried most of the sensilla, which included sensilla trichodea, sensilla chaetica, sensilla coeloconica, sensilla styloconica and sensilla auricillica. The observed morphological features showed sexual dimorphism.

Linear alkanes, carboxylic acids, aldehydes and unsaturated alcohols were found to be present in sex gland. GC-EAD response of male antennae to compounds in pheromone gland extracts showed two significant EAD active signals in which compound I evoked a stronger response in antennae than compound II.

This is the first study to describe the morphology of the antennal sensilla and to identify compounds as putative sex pheromone of H. ferrealis. Several chemoreceptive, mechanoreceptive and thermohygroreceptive sensilla and two active compounds were identified in this study. These analyses will provide a basis to better understand of the olfactory mechanisms for chemical communication between males and females and a useful foundation for future electrophysiological and behavioral studies.

Financing: FAPEAP – Amapa State Research Support Foundation.

Scholarship: CAPES-Coordination for the Improvement of Higher Education Personnel.